Antimicrobial Susceptibility Testing of Chlamydia trachomatis Using a Reverse Transcriptase PCR-Based Method
نویسندگان
چکیده
منابع مشابه
A reverse transcriptase-PCR based assay for in-vitro antibiotic susceptibility testing of Chlamydia pneumoniae.
Infections caused by Chlamydia spp are an important cause of human disease, and the accuracy and reproducibility of antimicrobial susceptibility tests for these bacteria could have considerable clinical implications. We have developed a reverse transcriptase PCR (RT-PCR) based method to determine the antibiotic susceptibility of Chlamydia spp., and compared this with conventional tests using im...
متن کاملScreening for Chlamydia trachomatis among Infertile Women Using PCR Method
Background & objectives: Chlamydia trachomatis is a gram negative bacterium and chlamydia infection, as a curable infection, is one of the most common sexually transmitted diseases (STD). With regard to the essential role of chlamydia in infertility, the study of the prevalence of asymptomatic cases is precious. The aim of this study was to determine of the prevalence of chlamydia trachomatis...
متن کاملPCR - Based Reverse Transcriptase Assay
ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
متن کاملThe antimicrobial susceptibility of Chlamydia trachomatis in cell culture.
The action of 22 antimicrobial agents against the SA2f strain of Chlamydia trachomatis has been studied by the use of a simple cell culture technique. Tests for bactericidal activity were undertaken with some of the agents, and latency of infection was demonstrated. The susceptibilities of 10 clinical strains of C. trachomatis and of SA2f to oxytetracyline, erythromycin, and spectinomycin in ce...
متن کاملIn Vitro Susceptibility Testing of Clinical Isolates of Chlamydia trachomatis
Penicillin class antibiotics have demonstrated varying degrees of in vivo and in vitro success when tested against Chlamydia trachomatis. The activity of ampicillin-sulbactam, an agent commonly utilized in the treatment of pelvic infections, was tested to ascertain if any antichlamydial activity is present. Up to six endocervical isolates of C. trachomatis were tested against each of five antib...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1999
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.43.9.2311